“ Evaluation of the effects and interactions of gas blending and feeding strategy on a

.................................................................................................. 15 CHAPTER 1. ANTIBODY PROCESSING................................................17 1.1. STRUCTURE AND USES OF ANTIBODIES.............18 1.2. PRODUCTION OF ANTIBODY FRAGMENTS..........22 1.3. EXPRESSION STRATEGIES USING E. coli.............24 1.4. FERMENTATION PROCESSING OF.........................30 Fab'ANTIBODY FRAGMENTS BY E. coli 1.5. DOWNSTREAM PROCESSING OF ANTIBODY......32 FRAGMENTS 1.6. METHODOLOGIES FOR THE IMPROVEMENT......34 OF FERMENTATION AND DOWNSTREAM PROCESSING 1.6.1. Scale-up and scale-down techniques.................35 1.6.2. Design of experiments methodology (DoE).......39 CHAPTER 2. DESIGN OF EXPERIMENTS 2.1. STATEMENT OF THE PROBLEM AND THE........... 43 PROJECT AIM 2.2. SELECTION OF FACTORS AND LEVELS...............45 2.2.1. Preliminary analysis............................................45